Quantitative Analysis of Corosolic Acid, a Type-II Anti-Diabetic Agent, in Different Parts of Lagerstroemia speciosa Linn.

An HPTLC method has been established for rapid and simple quantitative determination of corosolic acid, a type-II (non-insulin dependant) anti-diabetic agent found in the plant Lagerstroemia speciosa. To identify the high-yielding parts of the plant, different tissues, for example leaf, stem, stem bark, root, heartwood, calyx, and fruit were extracted with 80% ethanol. The extracts were chromatographed on aluminum plates coated with silica gel 60F254, with chloroform-methanol 8.5:1.5 (v/v) as mobile phase. Densitometric quantification of corosolic acid was performed in absorbance mode at 366 nm after derivatization. Amounts of corosolic acid in the different samples were calculated by use of the regression equations (r = 0.99603) of calibration plots, which showed there was a good linear relationship between peak area and amount of corosolic acid in the range 0.5–4.5 μg. The limits of detection (LOD) and quantitation (LOQ) were 6 and 16 ng, respectively. The method was validated for precision, repeatability, and accuracy. Recovery was determined by spiking samples with corosolic acid standard before and after extraction and found to be in the range 95.80–97.13%. The maximum corosolic acid content (0.89%) was found in the leaves.

References

  1. [1]T. Miura, N. Ueda, K. Yamada, M. Fukushima, T. Ishida, T. Kaneko, F. Matsuyama, Y. Seino, Biol. Pharm. Bull. 29 (2006) 585
  2. [2]W.V. Judy, S.P. Hari, W.W. Stogsdill, J.S. Judy, Y.M.A. Naguib, R. Passwater, J. Ethnopharmacol. 87 (2003)
  3. [3]M.C. Aguirre, C. Delporte, N. Backhouse, S. Erazo, M.E. Letelier, B.K. Cassels, X. Silva, S. Alegria and R. Negrete, Bioorg. Med. Chem. 14 (2006) 5673
  4. [4]Y. Yamaguchi, K. Yamada, N. Yoshikawa, K. Nakamura, J. Haginaka and M. Kunitomo, Life Sci. 79 (2006) 2474
  5. [5]K.S. Ahn, M.S. Hahm, E.J. Park, H.K. Lee and I.H. Kim, Planta Med. 64 (1998) 468.
  6. [6]M. Fukushima, F. Matsuyama, N. Ueda, K. Egawa, J. Takemoto, Y. Kajimoto, N. Yonaha, T, Miura, T. Kaneko, Y. Nishi, R. Mitsui, Y. Fujita, Y. Yamada, Y. Seino, Diabetes Res. Clin. Pract. 73 (2006) 174
  7. [7]M.K. Na, S.M. Yang, L. He, H. Oh, B.S. Kim, W.K. Oh, B.Y. Kim, J.S. Ahn, Planta Med. 72 (2006) 261
  8. [8]R.G. Udell, S.P. Hari, Corosolic acid formulation and its application for weight-loss management and blood sugar balance, United States Patent 6784206, 2004
  9. [9]V.U. Ahmad, Aatta-ur-Rahman, Hand Book of Nat. Prods. Data, Elsevier, Vol. 2, 1994, p. 800
  10. [10]K. Vijaykumar, P.B. Murthy, S. Kannababu, B. Syamasundar, and G.V. Subbaraju, Int. J. Appl. Sci. Engg. 2 (2006) 103
  11. [11]S.P. Ambasta (ed), The useful plants of India, Publication & Information Directorate, CSIR, 1992, p. 314
  12. [12]R. Sharma, Medicinal plants of India — An encyclopedia. Daya Publishing House, New Delhi, 2003, p. 144
  13. [13]A. Moffett, P. Shah, Pharmaceutical, therapeutic, and dietary compositions derived from Lagerstroemia speciosa L. plant, United States Patent 20060198907, 2006
  14. [14]Y. Suzuki, T. Unno, M. Ushitani, K. Hayasi, T. Kakuda, J. Nutr. Sci. Vit. 45 (1999) 791
  15. [15]T. Unno, I. Sakane, T. Masumizu, M. Kohno, T. Kakuda, Biosci. Biotech. Biochem. 61 (1997), 1772
  16. [16]U.V. Mallavadhani, G. Sahu, Chromatographia 58 (2003) 307
  17. [17]U.V. Mallavadhani, K.V.S. Satyanarayana, A. Mahapatra, Pharm. Biol. 42 (2004) 338
  18. [18]U.V. Mallavadhani, K.V.S. Satyanarayana, A. Mahapatra, A.V.S. Sudhakar, K, Narasimhan, J. Int. Plant Biol. 48 (2006) 1115
  19. [19]U.V. Mallavadhani, A.V.S. Sudhakar, K.V.S. Satyanarayana, A. Mahapatra, W. Li, R.B. van Breemen, Food Chem. 95 (2006) 58.

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