To evaluate the toxicity impact of ethanol concentrates of Banaba. This study utilized as a part of vivo methodologies. Harmfulness of Banaba concentrates was assessing through in vivo with diverse dosages. Thirty male grown-up Sprague-Dawley rats (10 weeks) measuring (180 to 200gm) separated into Control group I (Control-10% ordinary saline) and Treatment groups (500, 1000,2000 and 3000 mg/kg), n=6 for every gathering individually. The Banaba concentrates was offered orally to every rodent and disregard at 4 and 6 hourly for any physical change. In vivo biochemical analysis for the treated animal’ serum qualities were like control animal. Histological results demonstrated no periportal rot of the hepatocytes and no aggravation of lymphocytes and macrophages in both gatherings. No distinction was found in glomeruli or some other section of kidney tubules to that of control typical rats. Discoveries of the present study propose the Banaba are non-dangerous and suitable for further study.